Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing

US11085072B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• George M. Church · Brookline, US
• Evan R. Daugharthy · Cambridge, US
• Richard C. Terry · Carlisle, US
• Benjamin W. Pruitt · Cambridge, US
• Brian M. Turczyk · Peabody, US

Key dates

• Filing date: Feb 26, 2019
• Grant date: Aug 10, 2021
• Expiry date: Feb 26, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.