Systems and methods for performing digital PCR
US11085074B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 29, 2016 |
| Grant date | Aug 10, 2021 |
| Priority date | — |
| Expiry date | Jun 20, 2037 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0829
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.