Coherent anti-Stokes Raman scattering imaging method, and light source for the same

US11085824B2 · kind B2 · utility

Assignees

• CHONGQING INSTITUTE OF EAST CHINA NORMAL UNIVERSITY · CN
• EAST CHINA NORMAL UNIVERSITY · CN
• University of Shanghai for Science and Technology · CN

Inventors

• Heping Zeng · Chongqing, CN
• Kangwen Yang · Chongqing, CN
• Xu Chen · Apex, US
• Yue Shen · Hubei, CN
• Shikai Zheng · Chongqing, CN

Key dates

• Filing date: Sep 7, 2020
• Grant date: Aug 10, 2021
• Expiry date: Sep 7, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/653
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure provides a super-resolution fast-scanning coherent Raman scattering imaging method. The method: using pump light and Stokes light; combining the pump light and the Stokes light to obtain combined light; expanding/collimating the combined light; the combined light after the expanding/collimating entering a galvanometer, passing through a group of a scanning lens/a tube lens and being focused on a back focal plane of a microobjective and incidenting into a biological sample, such that the biological sample is excited to emit anti-Stokes light; collecting the excited anti-Stokes light by a detector. This method is characterized by deflecting, at different angles, a single light spot focused on the microobjective through a diffractive optics group including DOE and a dispersive element, into a plurality of 1×N light spots to incident into the biological sample, such that the anti-Stokes light excited from smaller molecules and being condensed and filtered, is collected by the detector.