Method and a kit for simultaneous analyses of thyroid hormones and related metabolites in serum
US11085936B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 2019 |
| Grant date | Aug 10, 2021 |
| Priority date | — |
| Expiry date | Mar 22, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2560/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and kit the simultaneous analysis of thyroid hormones (THs) and related metabolites in serum are disclosed. The method includes the extraction of THs and related metabolites, the derivatization of THs and related metabolites with 5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP), and the analysis of SPTPP derivatives using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Free or total THs (T3 and T4) together with their related metabolites (MIT, DIT, T0, T1, rT1, 3,3′-T2, 3,5-T2, and rT3) can be determined simultaneously, rapidly, and accurately. This invention overcomes the limitation that the traditional method can only be used for the analysis of T3 and T4 with low specificity. The invention exhibits extremely high analytical sensitivity for all of the target analytes (femtogram level). Moreover, this invention also possesses numerous other advantages, such as simple sample pretreatment, accurate determination, low cost, high efficiency, effective separation, and small sample amounts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.