Non-silencing selectable marker genes and methods of use

US11091774B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Mark A. Kay · Los Altos, US
• Jiamiao Lu · Mountain View, US

Key dates

• Filing date: May 2, 2018
• Grant date: Aug 17, 2021
• Expiry date: May 2, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/86
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided are nucleic acids and expression vectors having a non-silencing selectable marker gene, and methods of using the same. A subject expression vector includes an expression cassette and a non-silencing selectable marker gene. In some cases, the non-silencing selectable marker gene provides for drug resistance for prokaryotic cells, and includes a nucleotide sequence that (i) encodes a drug selectable marker protein; (ii) is operably linked to a promoter functional in prokaryotic cells, and (iii) includes an increased A/T content relative to a corresponding wild type nucleotide sequence. In some cases, the non-silencing selectable marker gene provides for drug resistance for prokaryotic cells, and includes a nucleotide sequence that (i) encodes a drug selectable marker protein; (ii) is operably linked to a promoter functional in prokaryotic cells, and (iii) has an A/T content in a range of from 52% to 70%.