Patent · US Active

Methods for hybridization based hook ligation

US11091791B2 · kind B2 · utility

1Cited by
2References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 23, 2018
Grant dateAug 17, 2021
Priority date—
Expiry dateApr 2, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6806
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Provided herein are compositions, methods, and kits for enriching for one or more nucleic acid sequences of interest in a sample. The methods include providing a circular ligase, one or more 5′ hook probes and/or one or more 3′ hook probes and contacting the sample comprising the nucleic acids with the circular ligase and one or more 5′ hook probes and/or one or more 3′ hook probes under conditions to allow the hook probes to selectively bind to the one or more nucleic acid sequences of interest, and under conditions to form one or more hook products, each hook product comprising the hook probes and the one or more nucleic acid sequences of interest.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.