Amplification with primers of limited nucleotide composition
US11091799B2 · kind B2 · utility
1Cited by
0References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 22, 2016 |
| Grant date | Aug 17, 2021 |
| Priority date | — |
| Expiry date | Jul 18, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/107
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of amplification from a single primer or a pair of forward and reverse primers of limited nucleotide composition. Limited nucleotide composition means that the primers are underrepresented in at least one nucleotide type. Such primers have much reduced capacity to prime from each other or to extend initiated by mispriming from other than at their intended primer binding sites in a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.