Optical method for tracking the in-vitro amplification of a nucleotide sequence
US11092530B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 15, 2018 |
| Grant date | Aug 17, 2021 |
| Priority date | — |
| Expiry date | Apr 19, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG03H2001/0447
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (10). The sample is placed between a light source (12) and an image sensor (16). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.