Method and device for label-free, high throughput holographic screening and enumeration of tumor cells in blood
US11092532B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 22, 2018 |
| Grant date | Aug 17, 2021 |
| Priority date | — |
| Expiry date | Jun 22, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG03H2001/0883
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention includes method and device for label-free holographic screening and enumeration of tumor cells in bulk flow comprising: a laser source, a micro-objective, a pinhole device and a collimating lens, a mirror, a sample chamber with a sample flow inlet on a first side of the sample chamber and a sample flow outlet connected by a microchannel, and a detector, wherein the collimated laser beam passes through microchannel and interacts with cells in the sample to generate a respective hologram at the detector, wherein a processor calculates a numerical reconstruction from the respective hologram and generates a focused image of the numerous cells using the numerical reconstruction, wherein the numerous cells are enumerated by looking at a size, a maximum intensity and a mean intensity of the focused image.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.