Patent · US Active

Method of differentiating pluripotent stem cells into mesenchymal stem cells under 3D spheroidal culture conditions

US11098281B2 · kind B2 · utility

0Cited by

1References

9Claims

0Family size

Assignee

University of Macau · CN

Inventors

Ren-He Xu · Madison, US
Bin Jiang · Fujian, CN
Li Yan · West Conshohocken, US

Key dates

Filing date	Jul 20, 2018
Grant date	Aug 24, 2021
Priority date	—
Expiry date	May 20, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2513/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A method of differentiating pluripotent stem cells into mesenchymal stem cells, a culture medium used in the method of differentiating pluripotent stem cells into mesenchymal stem cells and a method of performing tissue and organ regeneration by using the mesenchymal stem cells obtained by differentiation using the method of differentiating pluripotent stem cells into mesenchymal stem cells are provided. The method of differentiating pluripotent stem cells into mesenchymal stem cells comprises differentiating, completely under 3D suspension conditions, pluripotent stem cells into trophoblast-like cells using BMP4 and A8301, and then differentiating the trophoblast-like cells into mesenchymal stem cells. Neither of two differentiation processes needs passaging or replacement of a culture container.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.