Patent · US Active

17β-hydroxysteroid dehydrogenase mutants and application thereof

US11098287B2 · kind B2 · utility

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0References

9Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Zhiming Rao · Wuxi, CN
Minglong Shao · Wuxi, CN
Yetong Wang · Wuxi, CN
Yuling Wu · Wuxi, CN
Taowei Yang · Wuxi, CN
Xian Zhang · Beijing, CN
Meijuan Xu · Wuxi, CN

Key dates

Filing date	Nov 11, 2020
Grant date	Aug 24, 2021
Priority date	—
Expiry date	Nov 11, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y101/01051
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The disclosure discloses 17β-hydroxysteroid dehydrogenase mutants and application thereof, and belongs to the technical field of biology. The disclosure provides 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y with high specific enzyme activities, and the specific enzyme activities of the 17β-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y are as high as 1.85, 1.93, 2.06 and 5.15 U/mg, respectively, which are 1.11, 1.16, 1.24 and 3.10 times larger than that of wild-type 17β-hydroxysteroid dehydrogenase (1.66 U/mg).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.