High-throughput single-cell analysis combining proteomic and genomic information
US11098358B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Apr 30, 2020 |
| Grant date | Aug 24, 2021 |
| Priority date | — |
| Expiry date | Apr 30, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1402
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.
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