Immunoassay using at least two pegylated analyte-specific binding agents

US11099180B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Ralf Bollhagen · Penzberg, DE
• Barbara Upmeier · Iffeldorf, DE
• Werner Naser · Waldshut-Tiengen, DE
• Toralf Zarnt · Penzberg, DE

Key dates

• Filing date: Aug 1, 2019
• Grant date: Aug 24, 2021
• Expiry date: Aug 31, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/186
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure concerns a method and kits for measurement of an analyte in a microparticle-based analyte-specific binding assay. In the assay, the microparticles are coated with the first partner of a binding pair, mixing the coated microparticles and at least two analyte-specific binding agents, each conjugated to the second partner of the binding pair, and a sample suspected of containing the analyte. The second partner of the binding pair is bound to each of the analyte-specific binding agents via a linker comprising from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agents to the coated microparticles. The method also entails separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.