Linked perfusion to continuous-flow stirred-tank reactor cell culture system
US11104875B2 · kind B2 · utility
2Cited by
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19Claims
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Key dates
| Filing date | Jan 25, 2017 |
| Grant date | Aug 31, 2021 |
| Priority date | — |
| Expiry date | Nov 1, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.