Non-destructive seed genotyping

US11104895B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Kristof Van der Meeren · Zwijnaarde, BE
• Rainhard Koch · Leverkusen, DE
• Philipp Ellinger · Leverkusen, DE
• Nina Jansen · Leverkusen, DE
• Stephen Rae · Zevergem, BE
• Martin Clausen · Leverkusen, DE

Key dates

• Filing date: Mar 29, 2017
• Grant date: Aug 31, 2021
• Expiry date: Nov 10, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01015
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described herein are methods and means for reliably releasing maternal and/or paternal DNA from single seeds without substantially affecting the subsequent germination of the seeds. The methods are particularly suited for genome analysis of small grain cereals, including wheat. The methods may include enzymatic treatments for improved DNA recovery.