Method for isolating extracellular nucleic acids using anion exchange particles

US11104896B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Alexander Wolf · Oberkochen, DE
• Sandra Hammerschmidt · Hilden, DE
• Thorsten Voss · Leverkusen, DE

Key dates

• Filing date: Jun 10, 2016
• Grant date: Aug 31, 2021
• Expiry date: Jun 10, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.