Patent · US Active

Competitive compositions of nucleic acid molecules for enrichment of rare-allele-bearing species

US11104936B2 · kind B2 · utility

56Cited by
1References
20Claims
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Key dates

Filing dateOct 17, 2016
Grant dateAug 31, 2021
Priority date—
Expiry dateOct 24, 2038

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6806
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present disclosure describes the thermodynamic design and concentrations necessary to design probe compositions with desired optimal specificity that enable enrichment, detection, quantitation, purification, imaging, and amplification of rare-allele-bearing species of nucleic acids (prevalence <1%) in a large stoichiometric excess of a dominant-allele-bearing species (wildtype). Being an enzyme-free and homogeneous nucleic acid enrichment composition, this technology is broadly compatible with nearly all nucleic acid-based biotechnology, including plate reader and fluorimeter readout of nucleic acids, microarrays, PCR and other enzymatic amplification reactions, fluorescence barcoding, nanoparticle-based purification and quantitation, and in situ hybridization imaging technologies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.