Nucleated red blood cell warning method and device, and flow cytometer using the same
US11105742B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 30, 2017 |
| Grant date | Aug 31, 2021 |
| Priority date | — |
| Expiry date | Oct 4, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/014
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are nucleated red blood cell warning devices and methods, and flow cytometers using the same. The devices, methods and flow cytometers can warn whether nucleated red blood cells exist in a blood sample. The warning device may obtain forward-scattered light information, side-scattered light information and fluorescence information when cells in the blood sample pass through a detection region of the flow cytometer. The warning device may generate a side-scattered light-fluorescence dot plot, so as to classify leucocytes into four groups, and may generate a forward-scattered light-fluorescence dot plot, where the forward-scattered light-fluorescence dot plot can include a leucocyte group region. The warning device may obtain a predetermined feature region located at the left side of the leucocyte group region, perform statistics on the amount of characterization cells in the predetermined feature region, and provide a warning when the number of the characterization cells exceeds a threshold value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.