Method for preparing dual-functional hybrid thin-film for self-calibration detection of tumor-derived exosomes
US11105767B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 8, 2019 |
| Grant date | Aug 31, 2021 |
| Priority date | — |
| Expiry date | Nov 8, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/70596
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a dual-functional hybrid thin-film for self-calibration detection of tumor-derived exosomes is disclosed. A dual-functional hybrid thin-film, aptamer-BPNSs/Fc/ZIF-67/ITO, is constructed by facile self-assembly of a cobalt-based metal-organic framework (ZIF-67) composite doped with black phosphorus nanosheets (BPNSs), an aptamer and ferrocene (Fc) on an indium tin oxide (ITO) electrode. Methylene blue (MB) labeled aptamer specifically binds to CD63 protein to precisely capture protein. The protein is a specific biomolecule carried by breast cancer MCF-7 cell exosome, and realizes the detection of the tumor cell exosome. A self-calibration sensor for quantitative detection of the tumor exosome is constructed by using MB as a response signal and Fc as a reference. Compared with the prior art, the present invention features convenient operation, high sensitivity, low cost and excellent specificity, and can be used as a novel exosome self-calibration detection method for quantitative detection of the exosomes in biomedical samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.