Method and apparatus for injecting a chromatographic sample
US11105778B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 2017 |
| Grant date | Aug 31, 2021 |
| Priority date | — |
| Expiry date | Dec 11, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/347
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.