Ligand binding assays using MALDI-TOF mass spectrometry
US11105797B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 3, 2018 |
| Grant date | Aug 31, 2021 |
| Priority date | — |
| Expiry date | May 31, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2560/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for ligand binding assays includes an incubator that incubates a plurality of beads with a sample of interest wherein each of the plurality of beads comprises a tag of predetermined mass and a bait molecule. A washer washes the incubated beads so that weakly bound molecules are removed while strongly bound molecules are retained. A sample plate loader loads the washed beads into the sample plate such that respective ones of the plurality of beads are loaded into respective ones of the plurality of well. A sprayer deposits matrix assisted laser desorption ionization (MALDI) matrix material on the surface of the sample plate so that each of the plurality of beads is exposed to MALDI matrix material. A MALDI-TOF mass spectrometer receives the sample plate and performs mass spectrometry on samples in the plurality of wells. A computer executes an algorithm that analyzes the mass spectra.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.