Reagents for quantitative mass spectrometry

US11105810B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• DANA-FARBER CANCER INSTITUTE, INC. · US

Inventors

• Craig Braun · Newton, US
• Wilhelm Haas · Cambridge, US
• Steven P. Gygi · Cambridge, US
• Gregory H. Bird · Pelham, US
• Loren D. Walensky · Newton, US
• Martin Helmut Wuhr · Malden, US
• Brian K. Erickson · Brookline, US

Key dates

• Filing date: Nov 22, 2019
• Grant date: Aug 31, 2021
• Expiry date: Nov 22, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/15
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In some embodiments, a mass spectrometry tag may comprise a linker region, a mass balance region, and a reporter region. The mass spectrometry tag may be configured to fragment in a mass spectrometer via an energy dependent process to produce multiple reporter molecules. For example, the reporter region of the tag may be configured to produce at least two reporter molecules via fragmentation. In some embodiments, one or more regions of the tag may comprise at least one heavy isotope. In some such embodiments, the ability to fragment into multiple reporter molecules as well as the placement and/or number of heavy isotope(s) allows the mass spectrometry tag to be distinguished from other similar mass spectrometry tags. In some such embodiments, the ability to distinguish between tags having the same or substantially similar total mass to charge ratio and reporter region mass may allow the system to have a greater multiplexing capacity than conventional systems.