Methods for scarless introduction of targeted modifications into targeting vectors
US11111504B2 · kind B2 · utility
1Cited by
2References
29Claims
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Key dates
| Filing date | Apr 2, 2020 |
| Grant date | Sep 7, 2021 |
| Priority date | — |
| Expiry date | Apr 2, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for introducing a scarless targeted genetic modification into a preexisting targeting vector are provided. The methods can use combinations of bacterial homologous recombination (BHR) and in vitro assembly to introduce such targeted genetic modifications into a preexisting targeting vector in a scarless manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.