Patent · US Active

malR-knockout Bacillus licheniformis strain, construction method and use

US11111516B2 · kind B2 · utility

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4Claims

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Inventors

Shouwen Chen · 红钢城街道, CN
Fei Wu · Beijing, CN
Dongbo Cai · 红钢城街道, CN
Bowen Zhang · Newark, US
Junhui Li · Lishui, CN
Lijun Lou · Fujian, CN
Xiangqi Qiu · Fujian, CN

Key dates

Filing date	Aug 5, 2019
Grant date	Sep 7, 2021
Priority date	—
Expiry date	Aug 5, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/75
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided are malR-knockout Bacillus licheniformis strain, a construction method and a use. Using a genetic engineering method, gene, namely, the malR gene, which is in charge of conducting the transcription of the carbon metabolism transcription factor MalR, in the genome of Bacillus licheniformis DW2 is knocked out, thereby successfully obtaining Bacillus licheniformis DW2ΔmalR, from which the malR gene has been deleted. In comparison with Bacillus licheniformis DW2, the strain obtained by the construction can improve the Bacitracin yield in a fermentation broth during Bacitracin fermentation by at least 23%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.