Sequencing of nucleic acids via barcoding in discrete entities

US11111519B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Adam R. Abate · Cambridge, US
• John R. Haliburton · San Francisco, US
• Freeman Lan · San Francisco, US
• Adam R. Sciambi · San Francisco, US

Key dates

• Filing date: Feb 3, 2016
• Grant date: Sep 7, 2021
• Expiry date: Feb 3, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1497
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.