Multiple stage isothermal enzymatic amplification

US11118206B2 · kind B2 · utility

Assignee

• The Trustees of the University of Pennsylvania · US

Inventors

• Haim H. Bau · Swarthmore, US
• Michael G. Mauk · Wilmington, US
• Jinzhao Song · Philadelphia, US
• Changchun Liu · Sunnyvale, US

Key dates

• Filing date: Jan 13, 2017
• Grant date: Sep 14, 2021
• Expiry date: Oct 17, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/703
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods for multiple stage isothermal amplification of nucleic acid comprising a first substantially isothermal amplification reaction on the nucleic acid to generate a first amplification product and at least one substantially isothermal amplification reaction on the first amplification product to generate at least one second amplification product in an amount sufficient for recovery, testing, or characterization.