Thermal cycling methods and apparatuses for carrying out efficient polymerase chain reaction (PCR) processes to amplify deoxyribonucleic acid (DNA)
US11123739B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 15, 2017 |
| Grant date | Sep 21, 2021 |
| Priority date | — |
| Expiry date | Sep 21, 2039 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0838
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.