Single cell genomic sequencing using hydrogel based droplets
US11124830B2 · kind B2 · utility
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15Claims
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Key dates
| Filing date | Apr 15, 2020 |
| Grant date | Sep 21, 2021 |
| Priority date | — |
| Expiry date | Apr 15, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides ultrahigh-throughput single cell genomic sequencing methods, referred to herein as “SiC-seq”, which methods include encapsulating single cells in molten gel droplets to facilitate bulk cell lysis and purification of genomic DNA in microgels. Systems and devices for practicing the subject methods are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.