Patent · US Active

Applications of CRISPRi in high throughput metabolic engineering

US11130955B2 · kind B2 · utility

0Cited by

15References

30Claims

0Family size

Assignee

Zymergen Inc. · US

Inventors

Brian Chaikind · Oakland, US
Hendrik Marinus VAN ROSSUM · Oakland, US
Aaron Miller · Galveston, US
Paul Perkovich · Berkeley, US
Shawn Szyjka · Martinez, US
Kedar Patel · Fremont, US

Key dates

Filing date	Aug 15, 2019
Grant date	Sep 28, 2021
Priority date	—
Expiry date	Aug 15, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/20
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods, compositions, and kits for high throughput DNA assembly reactions in vitro. Modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.