Method for highly sensitive DNA methylation analysis
US11130991B2 · kind B2 · utility
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22Claims
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Key dates
| Filing date | Mar 8, 2018 |
| Grant date | Sep 28, 2021 |
| Priority date | — |
| Expiry date | Mar 8, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.