Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification

US11136360B2 · kind B2 · utility

Assignees

• TSINGHUA UNIVERSITY · CN
• South China University of Technology · CN

Inventors

• Zhanglin Lin · 流花街道, CN
• Tingting Wang · Nanhu, CN
• Qing Zhao · Shanghai, CN
• Xu Wang · 安丰镇, CN
• Bihong Zhou · 流花街道, CN
• Lei Xing · Palo Alto, US

Key dates

• Filing date: Dec 19, 2019
• Grant date: Oct 5, 2021
• Expiry date: Dec 19, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/92
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure describes a Mtu ΔI-CM intein variant containing one or more mutations or a biologically active fragment thereof, and a method for producing and purifying a molecule of interest using the intein variant. Further described are isolated fusion proteins comprising the intein variant and a tag and a molecule of interest. Also described are expression systems for expressing the intein variant as well as polypeptide screening methods employing the intein variant.