Compositions for enhancing targeted gene editing and methods of use thereof
US11136597B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 16, 2017 |
| Grant date | Oct 5, 2021 |
| Priority date | — |
| Expiry date | Dec 31, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3519
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Compositions and methods for enhancing targeted gene editing and methods of use thereof are disclosed. In the most preferred embodiments, gene editing is carried out utilizing a gene editing composition such as triplex-forming oligonucleotides, CRISPR, zinc finger nucleases, TALENS, or others, in combination with a gene modification potentiating agent such as stem cell factor (SCF), a CHK1 or ATR inhibitor, or a combination thereof. A particular preferred gene editing composition is triplex-forming peptide nucleic acids (PNAs) substituted at the γ position for increased DNA binding affinity. Nanoparticle compositions for intracellular delivery of the gene editing composition are also provided and particular advantageous for use with in vivo applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.