Live-cell seeding method for microarrays
US11136614B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 7, 2016 |
| Grant date | Oct 5, 2021 |
| Priority date | — |
| Expiry date | Apr 29, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56966
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.