Patent · US Active

Methods for preparing a sample for nucleic acid amplification using tagmentation

US11142786B2 · kind B2 · utility

14Cited by
1References
25Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 27, 2020
Grant dateOct 12, 2021
Priority date—
Expiry dateAug 27, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6846
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-continuing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, exposing the amplified nucleic acids to a solid surface, and clonally amplifying the amplified nucleic acids to generate clusters.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.