Patent · US Active

Nucleic acid sequencing with nanoscale electrode pairs

US11142793B2 · kind B2 · utility

3Cited by

38References

18Claims

0Family size

Assignee

PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

Stephen Turner · Menlo Park, US
Jeremiah Hanes · Woodside, US
Keith Bjornson · Fremont, US

Key dates

Filing date	Oct 30, 2019
Grant date	Oct 12, 2021
Priority date	—
Expiry date	Feb 19, 2040

Classification

Technology area (CPC G)Physics
CPC primaryG01N27/4146
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising two electrodes separated by an insulating layer are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the insulating region. A sequencing reaction mixture comprising nucleotide analogs having impedance labels is introduced to the array of nanoscale electronic elements under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.