Method for imaging a sample using a fluorescence microscope with stimulated emission depletion
US11143854B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 20, 2018 |
| Grant date | Oct 12, 2021 |
| Priority date | — |
| Expiry date | Dec 20, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for imaging a sample using a fluorescence microscope with stimulated emission depletion includes controlling the fluorescence microscope and an imaging process of the fluorescence microscope by a microscope controller. An overview image of a target region is generated with a second spatial resolution prior to the imaging process, the second spatial resolution being lower than a first spatial resolution used for scanning sample segments in the imaging process and higher than a third spatial resolution that has been adapted to an extent of an excitation light distribution. The overview image is analyzed to identify image regions without relevant image information. A radiant flux of the depletion light distribution is reduced within a scope of the imaging process when scanning sample segments which are assigned to the image regions without relevant image information.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.