Ascorbate peroxidase mutant MaAPXI-M36K and application thereof
US11149257B2 · kind B2 · utility
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Key dates
| Filing date | Dec 20, 2019 |
| Grant date | Oct 19, 2021 |
| Priority date | — |
| Expiry date | Feb 3, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y111/01011
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an ascorbate peroxidase mutant MaAPX1M36K and an application thereof, belonging to the technical field of biotechnology. An amino acid sequence of the mutant MaAPX1M36K of the present invention is shown in SEQ ID NO. 3. In the present invention, the mutant MaAPX1M36K reconstruction protein is obtained by the method of prokaryotic expression. It is found that the mutant MaAPX1M36K improves catalytic efficiency by nearly 5 times, providing a technical reference for further study and application of APX1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.