Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring
US11149266B2 · kind B2 · utility
2Cited by
8References
14Claims
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Key dates
| Filing date | Sep 13, 2017 |
| Grant date | Oct 19, 2021 |
| Priority date | — |
| Expiry date | May 21, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2523/307
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.