Nanopore protein conjugates and uses thereof

US11150233B2 · kind B2 · utility

Assignee

• Roche Sequencing Solutions, Inc. · US

Inventors

• Timothy Craig · Campbell, US
• Corissa Harris · Mountain View, US
• Matt Dipetro · Gilroy, US
• Mark Ambroso · Escondido, US
• Yaozhong Zou · Sunnyvale, US
• Marshall Porter · Santa Clara, US
• Issa Delkaninia · San Jose, US
• Alexxa Noble · San Jose, US
• James Fairman · Sunnyvale, US
• Wiseley Wu · Newark, US
• Kapil Bajaj · Fremont, US
• Giovanni Bellesia · San Jose, US
• Seong-Ho Shin · Hwaseong-si, KR

Key dates

• Filing date: Sep 28, 2018
• Grant date: Oct 19, 2021
• Expiry date: Jul 4, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/31
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.