Method of determining cell cycle stage distribution of cells
US11152197B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 24, 2015 |
| Grant date | Oct 19, 2021 |
| Priority date | — |
| Expiry date | Mar 10, 2036 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/40
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining a cell cycle stage distribution of cells includes the steps of providing a cell sample; pre-treating the cell sample with a solvent; mixing the pre-treated cell sample with a matrix solution to obtain a mixture solution; depositing the mixture solution on a sample plate; obtaining a mass spectrum analysis of the deposited mixture solution; and identifying at least two marker peaks from the mass spectrum analysis, wherein a ratio between the marker peaks provides information about a cell cycle stage distribution of the cell sample, wherein the mass spectrum analysis is a matrix-assisted laser desorption/ionization time-of-flight mass spectrum test.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.