Methods and uses of introducing mutations into genetic material for genome assembly

US11155806B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Solomon Endlich · Stanford, US
• Devin King · Los Angeles, US
• Ashby J. Morrison · Stanford, US

Key dates

• Filing date: Oct 28, 2019
• Grant date: Oct 26, 2021
• Expiry date: Oct 28, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of sequencing and assembling a nucleic acid sequence from a nucleic acid sample containing repetitive or low-information regions, which are typically difficult to sequence and/or assemble are provided. The methods of sequencing and assembling introduce mutations into the sample to increase sequence diversity between various repetitive regions present in the nucleic acid sample. This sequence diversity allows various segments to assemble independently of different, but similar sequences present in the nucleic acid sample.