Ketoreductase mutant and application thereof

US11162081B2 · kind B2 · utility

Assignee

• JILIN ASYMCHEM LABORATORIES CO., LTD. · CN

Inventors

• Hao Hong · Tianjin, CN
• Gage James · 梅厂镇, CN
• Jiangping Lu · Tianjin, CN
• Xingfu Xu · Tianjin, CN
• Wenyan Yu · Tianjin, CN
• Na Zhang · Nanhu, CN
• Yulei Ma · Tianjin, CN
• Yibing Cheng · Tianjin, CN
• Huiyan Mu · Tianjin, CN

Key dates

• Filing date: Jan 22, 2018
• Grant date: Nov 2, 2021
• Expiry date: Jan 22, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.