Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate

US11162082B2 · kind B2 · utility

Assignee

• METABOLIC EXPLORER · FR

Inventors

• An-Ping Zeng · Schwäbisch Hall, DE
• Chengwei MA · Hamburg, DE
• Yujun Zhang · Hamburg, DE

Key dates

• Filing date: Oct 10, 2018
• Grant date: Nov 2, 2021
• Expiry date: Oct 10, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y206/01052
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a genetically modified microorganism for the production of a derivative of 4-hydroxy-2-ketobutyrate selected in the group consisting of 1,3-propanediol, 3-hydroxypropionaldehyde, 3-hydroxypropionate and 2,4-dihydroxybutyrate, the microorganism further having a gene coding for a mutant phosphoserine aminotransferase having an improved L-homoserine aminotransferase activity converting by transamination L-homoserine into 4-hydroxy-2-ketobutyrate. Also provided is a method for the production of derivatives of 4-hydroxy-2-ketobutyrate selected in the group consisting of 1,3-propanediol, 3-hydroxypropionaldehyde, 3-hydroxypropionate and 2,4-dihydroxybutyrate by culturing the genetically modified microorganism in a culture medium and recovering the desired derivative.