Chloroplast targeting peptide sequence derived from nannochloropsis phosphoribulokinase and methods for use
US11162107B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 2017 |
| Grant date | Nov 2, 2021 |
| Priority date | — |
| Expiry date | Dec 5, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.