Process for the production of lipids from biomass employing oleaginous yeast

US11162118B2 · kind B2 · utility

Assignee

• ENI S.P.A. · IT

Inventors

• Roberta Miglio · Novara, IT
• Daniela Cucchetti · Busto Garolfo, IT
• Valentina RODIGHIERO · Milano, IT

Key dates

• Filing date: Apr 22, 2015
• Grant date: Nov 2, 2021
• Expiry date: Dec 2, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/30
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

Process for the production of lipids from biomass including at least one polysaccharide comprising: —subjecting said biomass to hydrolysis to obtain a mixture comprising a first solid phase and a first aqueous phase; —preparing an inoculum comprising at least one oleaginous microorganism in a first fermentation device to obtain a first fermentation broth; —feeding said first aqueous phase and said first fermentation broth to a second fermentation device to obtain a second fermentation broth; —subjecting at least a portion of said second fermentation broth to microfiltration to obtain a first retentate and a first permeate; —feeding said first retentate to said second fermentation device; —subjecting said first permeate to a purification treatment to obtain a second permeate and a second retentate; —feeding said second retentate to said second fermentation device; —at the end of said fermentation, subjecting said second fermentation broth to separation to obtain an aqueous suspension of oleaginous cellular biomass comprising lipids and a second aqueous phase. The lipids thus obtained can advantageously be used in the production of biofuels.