Detection of an amplification product using pH-sensitive dye
US11162133B2 · kind B2 · utility
5Cited by
2References
7Claims
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Key dates
| Filing date | May 12, 2017 |
| Grant date | Nov 2, 2021 |
| Priority date | — |
| Expiry date | Jun 11, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and Tris buffer in the range of 1.5 mM Tris to 5 mM Tris or equivalent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.