Using nucleic acid size range for noninvasive cancer detection

US11168356B2 · kind B2 · utility

Assignees

• The Chinese University of Hong Kong · CN
• GRAIL, INC. · US

Inventors

• Yuk-Ming Dennis Lo · Kowloon, CN
• Rossa Wai Kwun Chiu · Sha Tin, CN
• Kwan Chee Chan · Sha Tin, CN
• Peiyong Jiang

Key dates

• Filing date: Nov 1, 2018
• Grant date: Nov 9, 2021
• Expiry date: Jan 20, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Size-band analysis is used to determine whether a chromosomal region exhibits a copy number aberration or an epigenetic alteration. Multiple size ranges may be analyzed instead of focusing on specific sizes. By using multiple size ranges instead of specific sizes, methods may analyze more sequence reads and may be able to determine whether a chromosomal region exhibits a copy number aberration even when clinically-relevant DNA may be a low fraction of the biological sample. Using multiple ranges may allow for the use of all sequence reads from a genomic region, rather than a selected subset of reads in the genomic region. The accuracy of analysis may be increased with higher sensitivity at similar or higher specificity. Analysis may include fewer sequencing reads to achieve the same accuracy, resulting in a more efficient process.