Nanopores with internal protein adaptors
US11169138B2 · kind B2 · utility
17Cited by
41References
17Claims
0Family size
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Key dates
| Filing date | Apr 14, 2016 |
| Grant date | Nov 9, 2021 |
| Priority date | — |
| Expiry date | May 31, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6872
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting an analyte in a sample includes the steps of obtaining a nanopore sensor comprising a nanopore and a protein adaptor internalized in the lumen of the nanopore, adding a sample comprising an analyte to the cis side or the trans side of the nanopore, and measuring conductance across the nanopore. A change in conductance after adding the sample indicates the analyte is present in the sample and has bound to the protein adaptor. Nanopore sensors comprise a nanopore and a protein adaptor internalized in the lumen of the nanopore. The protein adaptor is a functional enzyme or ligand-binding protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.