Method and system for localisation microscopy
US11169368B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 2018 |
| Grant date | Nov 9, 2021 |
| Priority date | — |
| Expiry date | Dec 21, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B27/58
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Embodiments of the present invention provide a method and system for processing microscopy images to enable localisation analysis of high density raw data, and thereby achieve higher spatial resolution than would otherwise be the case. This is achieved by exploiting temporal redundancies in the image data resulting from close-to emitters that would otherwise be resolved as a single emitter were they to emit or fluoresce at the same time, but which, by virtue of emitting or fluorescing at slightly different (yet potentially overlapping) times, can be subject to temporal filtering by different filters of different temporal bandwidth to resolve the two emitters. Effectively, the different temporal filters have different time constants which work together to effectively highlight the different emission or fluorescence times of the two emitters, to thereby allow the two close-to emitters to be separately resolved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.