Antibodies and methods for the detection of cell death

US11175295B2 · kind B2 · utility

Assignee

• Defined Diagnostics, LLC · US

Inventors

• Jeffrey Allan Miller · Derry, US
• Eddie Phillip JEFFRIES · Sterling, US

Key dates

• Filing date: Jul 17, 2019
• Grant date: Nov 16, 2021
• Expiry date: Jul 17, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/7033
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein are antibodies having binding specificity to the amino acid sequences Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1) and Thr Val Glu Val Asp (SEQ ID NO:14), and methods of detecting cell death in a sample, comprising contacting the sample with a first antibody specific for a C-terminal amino acid sequence Ala Ser Ser Gly Leu Thr Val Glu Val Asp (SEQ ID NO:1) or Thr Val Glu Val Asp (SEQ ID NO:14) of a CK18 protein fragment having a C-terminal amino acid sequence of Val Glu Val Asp (SEQ ID NO:2) and a second antibody that specifically binds an epitope that is present in both full-length CK18 and the CK18 protein fragment, and that does not overlap with SEQ ID NO:1 or SEQ ID NO:14, under conditions such that the CK18 protein fragment present in the sample specifically binds to the first antibody and the second antibody, wherein one of the antibodies is bound to a solid support and the other antibody is bound to a detection moiety capable of producing a signal; optionally removing any unbound or excess material; and detecting the signal from the detection moiety, wherein the signal is positively correlated with the presence of the CK18 protein fragment in the sa…